E7 Mouse Monoclonal Antibody [Clone ID: 3D6F1]

Specifications

Product Data
Clone Name	3D6F1
Applications	WB
Recommend Dilution	ELISA: 1/10000.  Western Blot: 1/500 - 1/2000.
Reactivity	Human Papilloma Virus 16
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of E7 expressed in E. Coli.
Specificity	Recognizes HPV16 E7 (Human papillomavirus type 16 E7).
Formulation	State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P03129
Background	E7 transforming protein (Human papillomavirus type 16) has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcript.
Synonyms	Human Papilloma Virus Early protein 7
Reference Data

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