Siglec1 Rat Monoclonal Antibody [Clone ID: 3D6.112]

Specifications

Product Data
Clone Name	3D6.112
Applications	FC, IF, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1/50-1/100 (Fixation with either 2% paraformaldehyde or ethanol is recommended). Flow Cytometry: 1/100-1/1000. Immunocytochemistry.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Purified Sialoadhesin. Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.
Specificity	This antibody recognises Mouse CD169, a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Should this product contain a precipitate we recommend microcentrifugation before use. Avoid repeated freezing and thawing.
Gene Name	sialic acid binding Ig-like lectin 1, sialoadhesin
Database Link	Entrez Gene 20612 Mouse UniProt ID Q62230
Background	Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker; myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells; CD33, a myeloid differentiation antigen; and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.
Synonyms	Sialoadhesin, Siglec-1
Reference Data

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