LAMP1 Mouse Monoclonal Antibody [Clone ID: 4E9/11]

Specifications

Product Data
Clone Name	4E9/11
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of Neat antibody to label 10e6 cells in 100 µl (Membrane permeabilisation is required).
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine alveolar Macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
Specificity	This antibody is specific for Porcine CD107a, a cell surface antigen that is also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is expressed on macrophages and more weakly on monocytes and granulocytes.
Formulation	PBS, pH 7.4 Label: FITC State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q5K4G2
Background	Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.
Synonyms	LAMP-1, LAMPA, LGP120
Reference Data

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