IL2RA Mouse Monoclonal Antibody [Clone ID: K231.3B2]

Specifications

Product Data
Clone Name	K231.3B2
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Immunohistochemistry on Frozen Sections.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Con A activated porcine peripheral blood lymphocytes
Specificity	This antibody clone K231.3B2 recognizes porcine CD25, the alpha chain of the interleukin 2 receptor (IL-2Rα), also known as the low affinity Interleukin 2 receptor.  Clone K231.3B2 was clustered as CD25 at the First International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Lunney et al. 1994). Mouse anti pig CD25, clone K231.3B2 immunoprecipitates a protein of ~65-70kDa from activated lymphocyte preparations (Bailey et al. 1992).
Formulation	State: Purified State: Liquid Purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID O02733
Background	The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.
Synonyms	Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen
Reference Data

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