CD5 Mouse Monoclonal Antibody [Clone ID: 1H6/8]

Specifications

Product Data
Clone Name	1H6/8
Applications	FC, IP, WB
Recommend Dilution	Western Blot. Immunoprecipitation. Flow Cytometry: 1/10.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	ConA/PMA activated porcine peripheral blood cells.
Specificity	This antibody is specific for the CD5 cell surface antigen, which is expressed by porcine T lymphocytes. In pigs, CD5 can be used to distinguish between NK cells (CD4- CD8+ CD5-) and MHC-restricted cytotoxic T cells (CD4- CD8+ CD5+). The epitope recognised by this clone was designated CD5a at the Second International Swine Workshop.
Formulation	Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide, 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Database Link	UniProt ID Q9GMA0
Background	CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
Synonyms	CD5, LEU1
Reference Data

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