hnRNP K (HNRNPK) Mouse Monoclonal Antibody [Clone ID: F45 P9 C7]

Specifications

Product Data
Clone Name	F45 P9 C7
Applications	IHC, WB
Recommend Dilution	Western Blot: 1-2 µg/ml. Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Tissues: 1-5 µg/ml. Positive Controls: Colorectal cancer tissue, Calu6 and HT29 cell lysates.
Reactivity	Chicken, Human, Mouse, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the hnRNP-K protein and Mouse myeloma Ag8563 cells.
Specificity	This antibody reacts to Heterogeneous Nuclear Ribonucleoprotein K.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.08% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A/G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	51 kDa
Gene Name	heterogeneous nuclear ribonucleoprotein K
Database Link	Entrez Gene 3190 Human UniProt ID P61978
Background	Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.
Synonyms	HNRPK, Heterogeneous nuclear ribonucleoprotein K, TUNP
Reference Data

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