Myog Mouse Monoclonal Antibody [Clone ID: F12B]

Specifications

Product Data
Clone Name	F12B
Applications	ELISA, EMSA, IF, IHC, IP, WB
Recommend Dilution	ELISA. Gel Supershift. Immunohistochemistry on Formalin-fixed, Paraffin-embedded tissue sections (1-2 µg/ml). Immunofluorescence. Immunoprecipitation (2 µg/ml). Western Blot (1 µg/ml). Positive Control: Rhabdomyosarcoma and Wlim's tumors.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
Specificity	Recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin. This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.
Formulation	PBS containing 0.08% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	myogenin
Database Link	Entrez Gene 4656 HumanEntrez Gene 17928 MouseEntrez Gene 29148 Rat UniProt ID P20428
Background	Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
Synonyms	MYOG, MYF4, Myogenic factor 4, BHLHC3
Reference Data

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