RIT2 Mouse Monoclonal Antibody [Clone ID: 27G2]

Specifications

Product Data
Clone Name	27G2
Applications	IHC, WB
Recommend Dilution	Western Blot (0.5-1 µg/ml). Immunohistochemistry on Paraffin Sections.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Hybridoma produced by the fusion of splenocytes from mice immunized with full-length recombinant Human Rin protein and mouse myeloma cells.
Specificity	This antibody detects recombinant Rin protein expressed in 293 cells. Does not cross-react with Rit protein.
Formulation	20mM Sodium Phosphate, 150mM Sodium Chloride, 50% Glycerol at pH 7.5 and 3mM Sodium Azide as preservative. State: Purified State: Liquid (sterile filtered) purified IgG fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at -20°C. Avoid repeated freezing and thawing.
Gene Name	Ras like without CAAX 2
Database Link	Entrez Gene 6014 Human UniProt ID Q99578
Background	Rit and its neuron-specific homologue Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64% amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100% conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.
Synonyms	RIN, ROC2
Note	Predicted Molecular Weight: 36 kDa
Reference Data

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