Calpain (CAPN1+CAPN2) Mouse Monoclonal Antibody [Clone ID: 28F3]

Specifications

Product Data
Clone Name	28F3
Applications	IP
Recommend Dilution	ELISA: 0.5-1 ug/ml. Western blot: 0.5-1 ug/ml. Immunoprecipitation of both the native and denatured protein: 1-2ug/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Calpain purified from human placenta.
Specificity	This antibody specifically recognizes the 28-30 kD subunit of m-calpain and u-calpain. React with human and bovine calpains, but does not recognize calpains from rat or mouse.
Formulation	20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50% Glycerol and 3 mM Sodium Azide as preservative. Label: Agarose State: Liquid (sterile filtered) purified IgG fraction (> 95% pure).
Concentration	lot specific
Purification	Standard chromatographic techniques.
Conjugation	Agarose
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Background	The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, u-calpain is active at micromolar (uM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
Synonyms	CAPN2, CANPL2, CANP-2, CAPN1, CANPL1, CANP1
Reference Data

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