LEF1 (HMG Binding Domain) Mouse Monoclonal Antibody [Clone ID: REMB6]

Specifications

Product Data
Clone Name	REMB6
Applications	IF, WB
Recommend Dilution	Western Blot: 1-5 µg/ml Immunofluorescence on Paraformaldehyde-Fixed Cells: 1 µg/ml or 1/1000. Positive Control: Jurkat cell lysate.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length recombinant LEF-1 protein.
Specificity	REMB6 recognizes the High Mobility Group DNA domain of LEF-1.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.08% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	lymphoid enhancer binding factor 1
Database Link	Entrez Gene 51176 Human UniProt ID Q9UJU2
Background	Lymphoid Enhancer Factor-1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms.
Synonyms	Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha
Reference Data

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