Cd68 Mouse Monoclonal Antibody [Clone ID: ED1]

Specifications

Product Data
Clone Name	ED1
Applications	FC, IHC, IP, WB
Recommend Dilution	Immunohistochemistry on Paraffin Sections: 10 µg/ml (1/100), no antigen retrieval required. Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml (1/1000-1/2000).  Western Blot.  Immunoprecipitation.  FACS (preferably on permeabilized cells).  Suggested Positive Control: Rat spleen.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat spleen cells.
Specificity	This Monoclonal Antibody ED1 is useful for detecting Rat monocytes and macrophages and isolated dendritic (veiled) cells in the blood. The antibody recognises a single chain glycoprotein of 90-100kDa. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 has many characteristics in common with Mouse macrosialin and Human CD68.  Antigen, Epitope: CD68; ED1 recognises a 92kD cytoplasmic protein. The epitope has not been further characterized. Antigen Distribution: The antigen is found on 90% of monocytes in the peripheral blood. It is also expressed by 98% of isolated dendritic (veiled) cells.
Formulation	PBS, pH 7.2 State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.09% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	Cd68 molecule
Database Link	Entrez Gene 287435 Rat UniProt ID Q4FZY1
Background	The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
Synonyms	Gp110, Macrosialin, Macrophage marker
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: (The whole procedure is performed at room temperature) 1. Wash in PBS. 2. Block endogenous peroxidase. 3. Wash in PBS. 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber. 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber. 6. Wash in PBS. 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to rat) for 1h in a humid chamber. 8. Wash in PBS. 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS. 11. Counterstain with Mayer's hemalum.  Protocol with formalin-fixed, paraffin-embedded sections: (The whole procedure is performed at room temperature) 1. Deparaffinize and rehydrate tissue section. 2. Block endogenous peroxidase. 3. Wash in PBS. 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber. 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber. 6. Wash in PBS. 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to rat) for 1h in a humid chamber. 8. Wash in PBS. 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS. 11. Counterstain with Mayer's hemalum.
Reference Data

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