Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1F9]

Specifications

Product Data
Clone Name	1F9
Applications	ELISA, IP, WB
Recommend Dilution	Western blotting: 0.5 µg/ml for HRPO/ECL detection Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Positive control: phosphotyrosine MW standard (supplied with this antibody detection kit). Immunoprecipitation: use at 1 - 10 µg per 10e6 pervanadate-treated A431 cells. ELISA: 0.05 µg/ml.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to hemocyanine
Specificity	Clone 1F9 recognizes phosphotyrosine in the context of the surrounding amino acids, tolerating charged amino acids directly neighboured to phosphotyrosine. Epitope: ...P-E-pY-H-N...
Formulation	Lyophilized from 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent ultrafiltration and size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store the lyophilized antibody at -20°C. Upon reconstitution, aliquot and freeze in liquid nitrogen. Reconstituted antibody can be stored frozen at - 80°C.
Background	Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.
Reference Data

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