CD40 Mouse Monoclonal Antibody [Clone ID: HI40a]

Specifications

Product Data
Clone Name	HI40a
Applications	FC
Recommend Dilution	Flow Cytometry (effective for indirect immunofluorescence staining flow cytometry analysis using 0.25 μg/test).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human CD40a
Specificity	This antibody recognizes CD40 (BP50), a 48 kDa type I single chain transmembrane glycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiated plasma cells. CD40 antigen is also present on Hodgkin's and Reed-Sternberg cells, follicular dendritic cells, some macrophages, basal epithelial cells and endothelial cells.
Formulation	Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4 State: Azide Free State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein G chromatography (purity > 95% by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	CD40 molecule
Database Link	Entrez Gene 958 Human UniProt ID P25942
Background	CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.
Synonyms	TNFRSF5, CDw40, Bp50
Reference Data

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