CD11b (ITGAM) Mouse Monoclonal Antibody [Clone ID: MEM-170]

Specifications

Product Data
Clone Name	MEM-170
Applications	FC, FN
Recommend Dilution	Flow Cytometry: 1 µg/ml. Functional Application: The antibody MEM-170 blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human granulocytes
Specificity	The antibody recognizes CD11b antigen (Mac-1), a 165 kDa transmembrane protein type 1 mainly expressed on monocytes, granulocytes and NK-cells. The antibody blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.
Formulation	Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4 State: Azide Free State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein G affinity chromatography; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha M
Database Link	Entrez Gene 3684 Human UniProt ID P11215
Background	CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.