Interferon gamma (IFNG) Mouse Monoclonal Antibody [Clone ID: G-23]

CAT#: AM03102PU-N

Interferon gamma (IFNG) mouse monoclonal antibody, clone G-23, Aff - Purified



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CNY 3,140.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name G-23
Applications FC, WB
Recommend Dilution Flow Cytometry (2-4 μg/ml)
Positive Control: PMA/ionomycin stimulated Peripheral Blood Lymphocytes (PBL)
Application note: Intracellular staining
Western Blotting (1 μg/ml)
Positive control: recombinant human IFN-gamma.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Recombinant human IFN-gamma (aa 22-166 representing mature IFN-gamma).
Specificity The antibody G-23 reacts with IFN-gamma, a 20-25 kDa cytokine produced by activated Th1 cells and NK cells.
Formulation Phosphate buffered saline (PBS), pH~7.4 containing 15 mM Sodium Azide as preservative.
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration lot specific
Purification Affinity Chromatography on Protein A.
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name interferon, gamma
Background The Interferon gamma (IFN-gamma; 20-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN.
IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Synonyms IFN-gamma, gamma IFN
Reference Data
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Allograft rejection, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Proteasome, Regulation of autophagy, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Type I diabetes mellitus
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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