HLA Class II DR Mouse Monoclonal Antibody [Clone ID: MEM-12]

Specifications

Product Data
Clone Name	MEM-12
Applications	FC, IP, WB
Recommend Dilution	Flow Cytometry: 2 µg/ml. Positive Control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Immunoprecipitation: Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Western Blot (Non-Reducing Conditions): Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Do not heat/boil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Thymocyte membrane
Specificity	The antibody MEM-12 recognizes common epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; Dendritic cells, B Lymphocytes, Monocytes, Macrophages).
Formulation	PBS, pH 7.4 with 15 mM sodium azide as preservative State: Aff - Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	HLA DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of an alpha chain (36 kDa) and a beta chain (27 kDa). It is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response.
Synonyms	HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
Reference Data

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