CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: LT95]

Specifications

Product Data
Clone Name	LT95
Applications	FC
Recommend Dilution	Flow Cytometry: use 20 μl reagent / 100 μl of whole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	HUT-78 human T cell lymphoma cell line.
Specificity	The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
Formulation	Phosphate buffered saline (PBS) Label: PE State: Liquid purified IgG fraction. Stabilizer: 0.2% (w/v) high-grade BSA (Protease free) Preservative: 15 mM sodium azide Label: Conjugated with R-Phycoerythrin under optimum conditions
Purification	Size-exclusion chromatography.
Conjugation	PE
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	Fas cell surface death receptor
Database Link	Entrez Gene 355 Human UniProt ID P25445
Background	CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
Synonyms	FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
Reference Data

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