PRKACA Mouse Monoclonal Antibody [Clone ID: 6D2.1]

Specifications

Product Data
Clone Name	6D2.1
Applications	WB
Recommend Dilution	Suitable for Western Blotting (0.5-1 μg/ml). Positive control: HeLa human cervix carcinoma cell line Note: To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).
Specificity	This antibody strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.
Formulation	PBS, pH 7.4 with 15 mM sodium azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	lot specific
Purification	Protein A affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid freeze/thaw cycles.
Gene Name	protein kinase cAMP-activated catalytic subunit alpha
Database Link	Entrez Gene 5566 Human UniProt ID P17612
Background	Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.
Synonyms	PKA C-alpha
Reference Data

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