VCP Mouse Monoclonal Antibody [Clone ID: Hs-14]

Specifications

Product Data
Clone Name	Hs-14
Applications	FC, IF, WB
Recommend Dilution	Flow cytometry. Western blot. Immunocytochemistry: 10 μg/ml; Staining technique: Membrane permeabilization (acetone) is essential. Application note: The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought).
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Specificity	The antibody reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
Formulation	Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Precipitation methods and size-exclusion chromatography; purity > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	valosin containing protein
Database Link	Entrez Gene 7415 Human UniProt ID P55072
Background	VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Synonyms	15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein
Reference Data

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