VCP Mouse Monoclonal Antibody [Clone ID: Hs-14]

Specifications

Product Data
Clone Name	Hs-14
Applications	FC, IF
Recommend Dilution	Suitable for Flow Cytometry analysis of sperm cells using 20 μl reagent / 100 μl cell suspension. The content of a vial (1 ml) is sufficient for 50 tests.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Freshly ejaculated Human sperms were washed in PBS and extracted in 3% Acetic Acid, 10% Glycerol, 30mM benzaminidine. The acid extract was dialyzed against 0.2% Acetic Acid and subsequently used for immunization.
Specificity	The antibody reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
Formulation	Tris buffered saline (TBS) containing 15 mM Sodium Azide as preservative and 0.2% (w/v) high-grade BSA (protease free) as stabilizer Label: FITC State: Liquid purified Ig fraction Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Conjugation	FITC
Storage Condition	Store the antibody in the dark at 2-8°C. DO NOT FREEZE! Avoid prolonged exposure to light.
Gene Name	valosin containing protein
Database Link	Entrez Gene 7415 Human UniProt ID P55072
Background	VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Synonyms	15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein
Reference Data

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