Complement SC5b-9 (TCC / MAC) Mouse Monoclonal Antibody [Clone ID: 3R2/0]

Specifications

Product Data
Clone Name	3R2/0
Applications	ELISA, FC, IHC, WB
Recommend Dilution	ELISA. Western Blot. Flow Cytometry. Immunohistochemistry on Frozen Sections. Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognises the SC5b-9 complex of around 330 kDa.
Formulation	Borate buffered saline, pH8.4 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190 kDa C5b which is bound to cells via 71 kDa C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75 kDa S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation.
Synonyms	Terminal complement complex, Membrane attack complex
Note	Removal of Sodium Azide is recommended prior to use in functional assays.
Reference Data

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