Trypanosoma brucei Procyclin GPEET Mouse Monoclonal Antibody [Clone ID: 9G4]

Specifications

Product Data
Clone Name	9G4
Applications	ELISA, IF
Recommend Dilution	ELISA. Immunofluorescence.
Reactivity	Trypanosoma brucei
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant protein containing the pentapeptidyl repeat sequence of GPEET-PARP linked to the C-terminus of glutathione-S-transferase.
Specificity	This antibody recognizes Trypanosoma brucei procyclin GPEET.
Formulation	State: Ascites State: Lyophilized Ascitic Fluid Stabilizer: None Preservative: None
Reconstitution Method	Restore with 0.5 ml distilled sterile water.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q95PJ0
Background	GPEET and EP procyclins are the major surface glycoproteins of Trypanosoma brucei while it lives in the midgut (procyclic form) of tsetse flies (Glossina spp.). Trypanosoma brucei is transmitted between mammals by tsetse flies and the species causes African trypanosomiasis (or sleeping sickness). The surface of Trypanosoma brucei brucei insect forms is covered by an invariant protein coat consisting of procyclins. There are six or seven procyclin genes that encode unusual proteins with extensive tandem repeat units of glutamic acid (E) and proline (P) (referred to as EP repeats), and two genes that encode proteins with internal pentapeptide (GPEET) repeats. GPEET procyclin, is rich in Glu-Pro-Glu-Glu-Thr repeats. Although the EP forms of procyclins have been isolated and characterized by several laboratories, evidence for GPEET procyclin has largely been confined to the expression of its mRNA.
Reference Data

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