pan Dengue Virus Mouse Monoclonal Antibody [Clone ID: Dengue 1-11(3)]

Specifications

Product Data
Clone Name	Dengue 1-11(3)
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA. Western Blot (Reducing Conditions). Immunofluorescence. Immunohistochemistry on Paraffin Sections: Requires antigen retrieval using heat treatment prior to staining of paraffin Sections (Sodium citrate buffer pH 6.0 is recommended for this purpose).
Reactivity	Dengue Virus Types 1, Dengue Virus Types 2, Dengue Virus Types 3, Dengue Virus Types 4
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognizes all four dengue virus serotypes (DEN-1, DEN-2, DEN-3 and DEN-4) of the genus Flavivirus. Clone Dengue 1-11 (3) binds strongly to subtypes 1 and 2. It recognizes subtype 3 more weakly. Among the four subtypes, subtype 4 is recognized the least strongly and some customers have reported no recognition of subtype 4. The antibody detects an anti-E/envelope specific band of 61kDa under Reducing Conditions in Western blotting. A weak secondary band of 80kDa may also be apparent.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	The dengue virus is responsible for the tropical and sub-tropical diseases, dengue (DF) and dengue haemorrhagic fever (DHF), transmitted to individuals through the bite of the Aedes mosquito. The global distribution of dengue is expanding and comparable to that of malaria, with symptoms ranging from a mild viral syndrome of high fever, rash, headache, fatigue and muscle and joint pain, to the more severe and sometimes fatal DHF. Each dengue virus serotype is antigenically distinct, such that infection only provides an individual with immunity to the causative serotype, with the possibility of further infection by a different serotype or even multiple serotypes.
Reference Data

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