CD19 Mouse Monoclonal Antibody [Clone ID: Bu12]

Specifications

Product Data
Clone Name	Bu12
Applications	FC, IP
Recommend Dilution	Flow Cytometry: 1/50 - 1/200; 10µl of the suggested working dilution to label 1x10e6 cells in 100µl. Immunoprecipitation.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human EB-4 Burkitt lymphoma cell line.
Specificity	This antibody recognises CD19, a 95kD cell surface glycoprotein, which is expressed on cells of the B cell lineage and follicular dendritic cells but absent on plasma cells. CD19 is an important signal transduction molecule which is involved in the regulation of B lymphocyte development, activation and differentiation.
Formulation	Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide (NaN3) State: Purified State: Liquid purified Ig
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD19 molecule
Database Link	Entrez Gene 930 Human UniProt ID P15391
Background	CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12% of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.
Synonyms	Leu-12, B-cell marker
Reference Data

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