Tfrc Rat Monoclonal Antibody [Clone ID: R17217.1.4]
CAT#: AM00702RP-S
Tfrc rat monoclonal antibody, clone R17217.1.4, PE
Size: 300 ug
Conjugation: Unconjugated FITC
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CNY 4,510.00
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Specifications
Product Data | |
Clone Name | R17217.1.4 |
Applications | FC, IHC, IP |
Recommend Dilution | Flow Cytometry. |
Reactivity | Mouse |
Host | Rat |
Clonality | Monoclonal |
Specificity | Anti-mouse CD71 monoclonal antibody reacts with the transferrin receptor (CD71). The expression of CD71 is up-regulated after B cell or T cell activation. |
Formulation | PBS containing 0.09% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein Label: PE State: Liquid purified Ig fraction Label: R - Phycoerythrin |
Concentration | lot specific |
Conjugation | PE |
Storage Condition | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! |
Gene Name | transferrin receptor |
Database Link | |
Synonyms | TfR1, p90, Transferrin receptor protein 1 |
Note | Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1.0 μg of this antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 μl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: (Representative Histogram) Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 1.0 μg/10e6 cells Isotypic Control: PE Rat IgG2a |
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