Myog Mouse Monoclonal Antibody [Clone ID: F5D]

Specifications

Product Data
Clone Name	F5D
Applications	IF, IHC, WB
Recommend Dilution	Western Blot. Immunofluorescence. Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Rhabdomyosarcoma or Wilm's tumors.
Reactivity	Feline, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant protein containing Rat Myogenin amino acids 30-224
Specificity	Reacts with a 34 kD protein known as Myogenin. The antibody can immunoprecipitate protein-DNA complex. Cellular Localization: Nuclear.
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
Purification	Protein G Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	myogenin
Database Link	Entrez Gene 4656 HumanEntrez Gene 17928 MouseEntrez Gene 29148 Rat UniProt ID P20428
Background	Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Transfection of myogenin into multipotential mesodermal cells have been shown to convert the mesodermal cells to myoblasts. Expression of Myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
Synonyms	MYOG, MYF4, Myogenic factor 4, BHLHC3
Reference Data

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