HSF1 Rat Monoclonal Antibody [Clone ID: 4B4]

Specifications

Product Data
Clone Name	4B4
Applications	IF, IP, WB
Recommend Dilution	Western blot: 1µg/ml was sufficient for ECL immunoblot in 20µg of HeLa lysate. Immunofluorescence. Immunoprecipitation.
Reactivity	Human, Mouse, Rat
Host	Rat
Clonality	Monoclonal
Immunogen	Recombinant mouse HSF1 protein; epitope mapping to amino acids 378-395
Specificity	Highly specific for HSF1. Detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.
Formulation	PBS pH 7.4, 50% Glycerol, 0.09% Sodium azide State: Purified State: Liquid
Concentration	lot specific
Purification	Immunoglobulin fraction purified on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	heat shock transcription factor 1
Database Link	Entrez Gene 3297 Human UniProt ID Q00613
Background	HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents; binds to heat shock response elements (HSEs) in the upstream region of heat shock gene and activates their transcription. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA.
Synonyms	HSTF1, HSF-1
Reference Data

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