YES1 (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2B8]

Specifications

Product Data
Clone Name	2B8
Applications	ELISA, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use A431 untreated cell lysate as Positive Control. ELISA: 0.05 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH
Specificity	AM00159PU-N specifically recognizes Yes at 59 kDa.
Formulation	1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Gene Name	YES proto-oncogene 1, Src family tyrosine kinase
Database Link	Entrez Gene 7525 Human UniProt ID P07947
Background	Yes is a member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Yes, src and fyn are ubiquitously expressed while the other members are primarily expressed in hematopoietic cells. Each protein tyrosine kinase (PTK) contains three domain (SH1, SH2, SH3). The SH1 domain has PTK activity, while the SH2 and SH3 domains are invloved in mediating protein-protein interactions by binding phosphotyrosine- containing and proline-riche motifs. By means of SH2-phosphotyrosine binding, PTKs are able to associate with activated receptor protein tyrosine kinases, e.g. platelet-derived growth factor (PDGFR)- and epidermal growth factor (EGF)- receptor.
Synonyms	YES, p61-Yes, c-Yes
Note	Includes Positive Control A431 untreated cell lysate (See ''Protocols'' below). Protocol: Lyophilized Cell Lysate from serum starved A431 cells Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation! Aliquot and store frozen. Avoid repeated freeze/thaw cycles.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.