VASP pSer239 Mouse Monoclonal Antibody [Clone ID: 16C2]

Specifications

Product Data
Clone Name	16C2
Applications	FC, IF
Recommend Dilution	Flow Cytometry. Immunocytochemistry: 1-10 µg/ml (may tolerate 0.5% Formaldehyde fixation). For Immunoblotting and Immunoprecipitation use Cat.-No AM00156PU-N and AM00156BT-N.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH. Epitope: Phosphoserine 239
Specificity	This antibody recognizes VASP only, when Ser239 is phosphorylated, a site preferred by cGMP-dependent protein kinase (PKG) but also used by cAMP-dependent protein kinase (PKA). This antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, The antibody is able to monitor the phosphorylation state of VASP Serine239 as well as PKA activity.
Formulation	2 x PBS containing 0.09% Sodium Azide / PEG and Sucrose Label: FITC State: Liquid purified IgG fraction from serum-free cell culture supernatant Label: Fluorescein Isothiocyanate Molar radio: DOL = 2.9
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	FITC
Storage Condition	Aliquote and freeze (in liquid nitrogen at -20°C to -80°C). Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months. Avoid repeated freezing and thawing.
Predicted Protein Size	46/50 kDa
Gene Name	vasodilator-stimulated phosphoprotein
Database Link	Entrez Gene 7408 Human UniProt ID P50552
Background	VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines; particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament format
Synonyms	Vasodilator-stimulated phosphoprotein
Reference Data

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