VASP pSer157 Mouse Monoclonal Antibody [Clone ID: 5C6]

Specifications

Product Data
Clone Name	5C6
Applications	ELISA, FC, IF, IP, WB
Recommend Dilution	Western blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml. Immunoprecipitation: 1-10 µg per 10e6 pervanadate-treated A431 cells. Immunocytochemistry: 1-10 µg/ml (VASP-5C6 may tolerate 0.5% formaldehyde fixation). Flow Cytometry.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH.  Epitope: Phosphoserine 157
Specificity	This antibody recognizes VASP only, when Ser 157 is phosphorylated, a site preferred by cAMP-dependent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, antibody VASP-5C6 is able to monitor the phosphorylation state of VASP serine 157 as well as PKA activity.
Formulation	PBS with 0.09% Sodium Azide, PEG and Sucrose Label: Biotin State: Liquid purified IgG fraction from serum-free cell culture supernatant
Concentration	lot specific
Purification	Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Biotin
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	vasodilator-stimulated phosphoprotein
Database Link	Entrez Gene 7408 Human UniProt ID P50552
Background	VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines; particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament
Synonyms	Vasodilator-stimulated phosphoprotein
Reference Data

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