SRC pTyr416 Mouse Monoclonal Antibody [Clone ID: 9A6]

Specifications

Product Data
Clone Name	9A6
Applications	ELISA, FC, IF, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjuagted to KLH Epitope: phosphotyrosine 416
Specificity	AM00145PU-N specifically interacts with activated src phosphorylated at Tyr 416 and with src family kinases phosphorylated at the corresponding Tyr in the activation loop. The antibody does not crossreact with the non-phosphorylated form of src nor with unrelated phosphorylation sites.
Formulation	2 x PBS / 0.09% Sodium Azide / PEG and Sucrose. State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1.0 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freezing and thawing.
Gene Name	SRC proto-oncogene, non-receptor tyrosine kinase
Database Link	Entrez Gene 6714 Human UniProt ID P12931
Background	Src is the best understood member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Src is activated by PDGF and other growth factors that activate transmembrane tyrosine kinase receptors as well as increased intracellular calcium or oxidative stress. Src becomes activated upon dephosphorylation of Tyr 527, leading to a conformational change of the src molecule towards an "open", active conformation which allows binding to other proteins via the SH2 and SH3 domain. Activated src phosphorylates a variety of substrates involved in the mitogenic signalling pathway, cytoskeletal organization, cell-substrate adhesion, cell-cell adhesion, cell-cell communication, and RNA processing.
Synonyms	c-Src, pp60c-src
Note	Molecular weight of antigen: 60 kDa
Reference Data

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