p38 (MAPK14) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13D5]

Specifications

Product Data
Clone Name	13D5
Applications	ELISA, IF, IP, WB
Recommend Dilution	Western Blot: 1 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml. Immunoprecipitation: 1 - 10 µg per 106 pervanadate-treated A431 cells. Immunocytochemistry.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH
Specificity	This antibody specifically interacts with the N-terminus of SAPK2 / p38 / CSBP.
Formulation	1 ml PBS / 0.1 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	mitogen-activated protein kinase 14
Database Link	Entrez Gene 1432 Human UniProt ID Q16539
Background	Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. Stress-activated Protein Kinase 2 (SAPK2/p38) phosphorylates conserved Ser - Pro or Thr - Pro peptide motifs.
Synonyms	Mitogen-activated protein kinase 14, p38 alpha, MXI2, SAPK2A, CSBP, CSBP1, CSBP2, CSPB1
Note	Molecular Weight: 38kDa. Epitope: N-terminus, interacts with SAPK2 (p38/CSBP).
Reference Data

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