RAF1 pSer621 Mouse Monoclonal Antibody [Clone ID: 6B4]

Specifications

Product Data
Clone Name	6B4
Applications	ELISA, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH. Epitope: Phosphoserine 621
Specificity	This antibody Clone 6B4 specifically recognizes c-raf phosphorylated at Serine 621. The antibody is an important tool to analyse the contribution of the Serine 621 phosphorylation site to c-raf activity.
Formulation	1ml 2 x PBS containing 0.09% Sodium Azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction from serum-free cell Culture Supernatant
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Predicted Protein Size	74 kDa
Gene Name	Raf-1 proto-oncogene, serine/threonine kinase
Database Link	Entrez Gene 5894 Human UniProt ID P04049
Background	The c-raf protein is an important kinase in the transmission of extracellular stimuli to the nucleus. Mitogenic stimulaton of cells induces hyperphosphorylation of c-raf in multiple serine, threonine and tyrosine residues. Phosphorylation of serines 43, 259 and 621 has been implicated in the negative regulation of raf. Phosphorylation of serine 43 interferes with ras binding, serine 259 and serine 621 represent binding sites for the 14-3-3 proteins.
Synonyms	C-RAF, Raf-1, cRaf
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway

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