PYK2 (PTK2B) pTyr402 Mouse Monoclonal Antibody [Clone ID: 14F6]

Specifications

Product Data
Clone Name	14F6
Applications	ELISA, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 µg/ml.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH
Specificity	This antibody specifically interacts with activated Pyk2/CAKβ phosphorylated at tyrosine 402. The antibody does not crossreact with the non-phosphorylated form of Pyk2/CAKβ nor with unrelated phosphorylation sites.
Formulation	1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	protein tyrosine kinase 2 beta
Database Link	Entrez Gene 2185 Human UniProt ID Q14289
Background	Proline-rich tyrosine kinase 2 (Pyk2; also known as cellular adhesion kinase β, CAK β) is a 116 kDa cytoplasmic tyrosine kinase. Pyk2 is rapidely phosphorylated on tyrosine residues in response to activation by G-protein-coupled receptors as well as stress signals. Phosphorylation of Pyk2 leads to activation of stress-activated kinase 1 and 2 (SAPK1 and SAPK2).
Synonyms	PYK2, RAFTK, Protein tyrosine kinase 2 beta, Focal adhesion kinase 2, CAK-beta, CADTK
Note	Molecular Weight: 116kDa Epitope: phosphotyrosine 402 S D I pY A E I
Reference Data

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