Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 16F4]

Specifications

Product Data
Clone Name	16F4
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: Use at 0.05 µg/ml. Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: Use at 1-10 µg per 10e6 pervanadate-treated A431 cells. Included Positive Control: Phosphotyrosine MW standard.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to KLH Epitope: ... D-I-pY-A-E ...
Specificity	This antibody recognizes Phosphotyrosine in the context of the surrounding amino acids, tolerating hydrophobic amino acids directly neighboured to Phosphotyrosine.
Formulation	PBS with 0.09% Sodium Azide/PEG and Sucrose. Label: Biotin State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Biotin
Storage Condition	Aliquote and freeze in liquid nitrogen. Antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Background	Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.
Reference Data

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