Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2C8]

Specifications

Product Data
Clone Name	2C8
Applications	ELISA, IP, WB
Recommend Dilution	Western Blot: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunoprecipitation: 1 - 10 µg per 106 pervanadate-treated A431 cells.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH
Specificity	Mab PTYR-2C8 recognizes a broad range of tyrosine--phosphorylated proteins in crude cell extracts and may therefore be particularly well-suited for the detection/screening of tyrosine phosphorylated proteins.
Formulation	PBS/0.09% Na-Azide/PEG and Sucrose Label: Biotin State: Liquid purified IgG
Concentration	lot specific
Purification	Size exclusion chromatography
Conjugation	Biotin
Storage Condition	Store the antibody at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Background	Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.
Reference Data

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