LEF1 Mouse Monoclonal Antibody [Clone ID: 2D12]

Specifications

Product Data
Clone Name	2D12
Applications	ELISA, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant LEF
Specificity	This antibody specifically recognizes LEF at 55-57 kDa. It does not crossreact with TCF3 and TCF4.
Formulation	PBS/0.09% Na-Azide/PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified Ig
Concentration	lot specific
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	lymphoid enhancer binding factor 1
Database Link	Entrez Gene 51176 Human UniProt ID Q9UJU2
Background	LEF is a transcription factor of the LEF/TCF protein family. LEF interacts with cytoplasmic beta-catenin and regulates the expression of genes containing a LEF/TCF binding site. The formation of LEF - beta-catenin heterodimers is an important step in the wnt/wg signal transduction pathway.
Synonyms	Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha
Reference Data

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