JNK1 (MAPK8) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5D10]

Specifications

Product Data
Clone Name	5D10
Applications	ELISA, IHC, WB
Recommend Dilution	Western Blot: 0.5 μg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.05 μg/ml. Immunohistochemistry on frozen sections.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH.
Specificity	This antibody specifically interacts with the N-terminus of SAPK1γ/jnk1 kinases.
Formulation	1 ml 2 x PBS / 0.1 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	mitogen-activated protein kinase 8
Database Link	Entrez Gene 5599 Human UniProt ID P45983
Background	Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines.The SAPK1/jnk family consists of 3 isoforms: SAPK1α/jnk2; SAPK1β/jnk3; SAPK1γ/jnk1.
Synonyms	Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8
Reference Data

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