JNK2 (MAPK9) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12C5]

CAT#: AM00079PU-N

JNK2 (MAPK9) (incl. pos. control) mouse monoclonal antibody, clone 12C5, Purified

Conjugation: Unconjugated Biotin



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CNY 5,148.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 12C5
Applications ELISA, IHC, WB
Recommend Dilution Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.05 g/ml.
Immunohistochemistry.
Included Positive Control: Cell lysate from untreated A431 cells (See Protocol).
Reactivity Canine, Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Synthetic peptide conjugated to KLH.
Epitope: N-terminus
Specificity This antibody specifically interacts with the N-terminus of SAPK1α/jnk2 kinases.
Formulation 1 ml PBS containing 0.09% Sosium Azide, PEG and Sucrose
State: Purified
State: Lyophilized purified IgG fraction.
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Subsequent thiophilic adsorbtion and Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size 54 kDa
Gene Name mitogen-activated protein kinase 9
Background Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1α/jnk2; SAPK1β/jnk3; SAPK1γ/jnk1.
Synonyms JNK-55, PRKM9, MAP kinase 9
Note Protocol: Positive Control Cell Lysate (A431 Untreated)

Formulation: Lyophilized cell lysate from serum starved A431 Cells.

Reconstitution
:
 Restore by Addition of 200 µl Water. After complete solubilization Add 200 µl 2x SDS-PAGE Sample Buffer, mix and incubate at 90°C for 5 mn.

Storage
:
Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.
Shelf life: one year from despatch.

Application
:
The Positive Control is recommended for Immunoblot (20 µl of Positive Control Cell lysate corresponds to ~ 20.000 Cells).
Use µl /lane (mini gel) for HRPO/ECL detection of the target proteins.

Please Note:
The lyophilized cell lysates contain SDS and are not recomended for the applications with native proteins such as Immunoprecipitation.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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