Insulin Receptor (INSR) (beta chain) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 9H4]

CAT#: AM00077PU-N

Insulin Receptor (INSR) (beta chain) (incl. pos. control) mouse monoclonal antibody, clone 9H4, Purified



Need it in bulk or conjugated?
Get a free quote

CNY 5,973.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 9H4
Applications ELISA, IHC, WB
Recommend Dilution ELISA: 0.1 µg/ml.
Immunohistochemistry on Frozen Sections.
Western Blot: 1 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Included Positive Control: Cell lysate from untreated T47D cells.
Positive Control: Cell lysate from untreated T47D cells.
Format: Lyophilized cell lysate from Serum starved T47D cells
Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Please NOTE: The lyophilized cell lysates contains SDS and are not recommended for applications with native proteins such as in immunoprecipitation.
Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reactivity Canine, Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Synthetic peptide conjugated to KLH.
Epitope: Kinase Activation Loop
Specificity This antibody specifically recognizes the activation loop of Insulin receptor (phosphorylation-independent).
Formulation 1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name insulin receptor
Background The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.
Synonyms Insulin Receptor
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...