Insulin Receptor (INSR) pTyr1150/1151 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10C3]

Specifications

Product Data
Clone Name	10C3
Applications	ELISA, WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from insulin-treated HEK-293 cells. ELISA: 0.1 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phosphopeptide conjugated to KLH.  Epitope: Phosphotyrosine 1150/1151 E T D pY pY R K
Specificity	This antibody recognizes Insulin receptor phosphorylated at tyrosine residues 1150/1151 and also the IGF1 receptor.
Formulation	PBS containing 0.09 Sodium Azide/PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	97 kDa
Gene Name	insulin receptor
Database Link	Entrez Gene 3643 Human UniProt ID P06213
Background	The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.
Synonyms	Insulin Receptor
Note	Protocol: Positive Control Provided: 293 Insulin treated Positive Control Cell lysate Format: Lyophilized cell lysate from HEK - 293 cells.  Serum starved cells were treated for 15 min with Insulin. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.  Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.  Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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