GSK3 beta (GSK3B) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11B9]

Specifications

Product Data
Clone Name	11B9
Applications	ELISA, WB
Recommend Dilution	ELISA: Use at 0.05 µg/ml. Western Blot (Immunoblotting): 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Provided Postive Control: Cell lysate from untreated HepG2 cells (See Protocols for more details).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to KLH. Epitope: N-terminal region of GSK3beta
Specificity	This antibody specifically recognizes GSK3beta at 46 kDa and does not crossreact with GSK3alpha.
Formulation	PBS containing 0.09% Sodium Azide, PEG and Sucrose. Label: Biotin State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody (aliquote in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Predicted Protein Size	46 kDa
Gene Name	glycogen synthase kinase 3 beta
Database Link	Entrez Gene 2932 Human UniProt ID P49841
Background	Glycogen Synthase Kinase 3 beta (GSK3beta) is a serine/threonine kinase that is acting independently of most signal transduction pathways. The activity of GSK3beta can be modulated by phosphorylation of Ser9 and Tyr215 in the activation loop. GSK3beta plays a crucial role in phosphorylation of beta-catenin and thus is an important kinase involved in the regulation of beta-catenin/wnt-signaling.
Synonyms	GSK-3 beta, Glycogen synthase kinase-3 beta, GSK3B
Note	Protocol: Positive Control: Cell lysate from untreated HepG2 cells Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing
Reference Data

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