Her2 (ERBB2) pTyr1248 Mouse Monoclonal Antibody [Clone ID: 6G7]

Specifications

Product Data
Clone Name	6G7
Applications	WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to hemocyanin
Specificity	This antibody recognizes erbB2 phosphorylated at tyrosine 1248 at 185 kDa and might crossreact with EGFR.
Formulation	1 ml PBS / 0.09 % Na-azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size exclusion chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	erb-b2 receptor tyrosine kinase 2
Database Link	Entrez Gene 2064 Human UniProt ID P04626
Background	ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of downstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248.Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of Thr 686 and Ser 1113 interferes with erbB2 endocytosis and degradation.
Synonyms	HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19
Reference Data

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