Her2 (ERBB2) pThr686 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7F8]

Specifications

Product Data
Clone Name	7F8
Applications	WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from PMA-/pervanadate-treated A431 cells (See Protocols for more details).
Reactivity	Canine, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to KLH. Epitope: Phosphothreonine 686
Specificity	This antibody specifically recognizes Human erbB2 phosphorylated at Thr686.
Formulation	PBS / 0.09% Sodium Azide / PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	erb-b2 receptor tyrosine kinase 2
Database Link	Entrez Gene 2064 Human UniProt ID P04626
Background	ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of downstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248.Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of Thr 686 and Ser 1113 interferes with erbB2 endocytosis and degradation.
Synonyms	HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19
Note	Protocol: Positive Control: Cell lysate from A431 pervanadate + PMA treated Format: Lyophilized cell lysate from A431 cells. Serum starved cells were treated for 15 min. with pervanadate plus PMA. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing
Reference Data

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