EGFR (960-980) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 16F8]

Specifications

Product Data
Clone Name	16F8
Applications	WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Positive control: Cell lysate from untreated HepG2 cells (included - please see "Protocols"). Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to KLH.
Specificity	This antibody specifically recognizes the cytoplasmic domain of EGF receptor (aa 960 - 980). Recognition is independent of the phosphorylation status.
Formulation	PBS/0.09% Na-Azide/PEG and Sucrose Label: Biotin State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Subsequent ultrafiltration and size exclusion chromatography
Conjugation	Biotin
Storage Condition	Aliquote and store the antibody (in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y845 by src family kinases. Phosphorylation of Y1045 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1047 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T654 (by PKC) and T669 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note	A positive control cell lysate (HepG2 untreated) is included. For technical details please see "Protocols". Protocol: Positive Control Cell Lysate: HepG2 Untreated Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Applications: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80,000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation. Storage: Aliquote and store frozen. Avoid repeated freezing and thawing.
Reference Data

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