EGFR pTyr869 Mouse Monoclonal Antibody [Clone ID: 12A3]

Specifications

Product Data
Clone Name	12A3
Applications	IF
Recommend Dilution	Immunocytochemistry: Use at 1-10 µg/ml Use AM00031PU-N or AM00031BT-N for Immunoblotting and Immunoprecipitation applications.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to KLH
Specificity	Clone 12A3 specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Antibody AM00031FC-N does not crossreact with the highly homologues pTyr877 of activated erbB2.
Formulation	PBS containing 0.09% Sodium Azide / PEG and Sucrose. Label: FITC State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Conjugation	FITC
Storage Condition	Aiquote and freeze in liquid nitrogen. Antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed Aliquots may be stored at 4°C up to 3 months.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 13649 MouseEntrez Gene 1956 Human UniProt ID P00533
Background	EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Note	Mol. weight of antigen: 180 kD
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
Protein Pathways	Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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