N Cadherin (CDH2) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7E9]

CAT#: AM00013PU-N

N Cadherin (CDH2) (incl. pos. control) mouse monoclonal antibody, clone 7E9, Purified

Conjugation: Unconjugated Biotin



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Clone Name 7E9
Applications WB
Recommend Dilution Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Included Positive Control: Cell lysate from untreated SH-SY5Y cells (See Ptotocols for more details).
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Peptide conjugated to hemocyanin.
Epitope: Cytoplasmic domain
Specificity This antibody specifically recognizes N-Cadherin at 127 kDa.
Formulation 1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose
State: Purified
State: Lyophilized purified IgG fraction.
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation Unconjugated
Storage Condition Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Avoid repeated freezing and thawing.
Gene Name cadherin 2
Background Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal), and M- (muscle) cadherin.
Synonyms CDH2, CDHN, NCAD, Neural cadherin, CDw325, Cadherin N, N-Cadherin
Note Protocol: Positive Control: Cell lysate from untreated SH-SY5Y cells

Format: Lyophilized cell lysate from serum starved SH-SY5Y cells.

Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.

Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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