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Lentiviral Vectors as Catalysts for Vaccine Development

Jun 16, 2023

In the ever-evolving landscape of genetic research, lentiviral gene delivery has emerged as a powerful tool that has revolutionized the field of gene therapy and vaccine development. Lentiviral vectors (LVs), derived from HIV-1, have gained significant attention due to their unique ability to efficiently and stably transfer genetic material into both dividing and non-dividing cells. Particularly in the realms of infectious diseases and immuno-oncology, lentiviral vectors have gained substantial attention by effectively transducing dendritic cells (DCs) in vivo.

Recent studies have unveiled the ability of lentiviral vectors to induce endogenous gene expression of antigens within DCs, facilitating direct antigen presentation. This advantageous feature distinguishes lentiviral vectors from adenoviral vectors, eliminating the need for cross presentation and external antigen capture.

A study conducted in 2023 by Kirill Nemirov et al., demonstrated the remarkable capability of GFP lentiviral vectors, to transduce different subtypes of DCs in vivo, triggering the expansion of naïve T cells2. These subtypes include:

  • Myeloid
    • CD11c+
    • CD11b+
    • CD8−
  • Lymphoid
    • CD11c+
    • CD11b-
    • CD8+
  • Plasmacytoid
    • CD11cint
    • B220+

Excitingly, numerous clinical trials are currently underway, evaluating the therapeutic efficacy and safety of lentiviral-based gene therapy in the treatment of diseases such as ADA-SCID3 , as well as infectious diseases like Flavivirus, COVID-19, and Tuberculosis1.

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Lentiviral Validation Data

2x10^4 293T cells per well of 96-well plate were transduced with different volumes of Lenti-GFP particles ([PS100071V]: Lenti-C-mGFP) (3.5x10^7 TU/ml). Particles were produced using Lenti-vpak packaging kit ([TR30037]). Fluorescent images were taken 72 hours post transduction.
View more Validation Data>>

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Citations

  1. Lundstrom K. Viral Vectors in Gene Therapy: Where Do We Stand in 2023? Viruses. 2023 Mar 7;15(3):698. doi: 10.3390/v15030698. PMID: 36992407; PMCID: PMC10059137.
  2. Nemirov K, Bourgine M, Anna F, Wei Y, Charneau P, Majlessi L. Lentiviral Vectors as a Vaccine Platform against Infectious Diseases. Pharmaceutics. 2023 Mar 5;15(3):846. doi: 10.3390/pharmaceutics15030846. PMID: 36986707; PMCID: PMC10053212.
  3. https://clinicaltrials.gov/ct2/show/NCT01380990

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