Primer Pairs and Standard FAQs

qSTAR qPCR Primer Pairs are pre-designed, qPCR tested and ready-to-use primer sets for SYBR Green based qPCR experiments. The primer pairs are also available in a 96-well panel format covering broad range of pathways for cancer biomarker profiling.

No. This kit has not been designed to accommodate any commercial probe based qPCR.

The Tm of a qSTAR qPCR Primer is around 60, and the amplicon is around 95 to 140 bp.

Whenever possible, an amplicon by a qSTAR qPCR Primer Pair covers exon junction or junctions.

Yes, we can provide our primer panels in other plates within approximately one week of receiving the custom order. Please email the catalog numbers of the qPCR primer panels needed plus the catalog number and manufacturer of the plates to techsupport@origene.com. Our technical support scientists will prepare a custom quote that can be used to order custom panels.

Yes, OriGene can design a custom qPCR primer panel. Custom panels are offered for human and mouse genes in 200 reactions format for purchase, delivered in 2-D bar code matrix tubes.

Each panel is delivered with a USB drive that contains a spreadsheet of primer sequences and locations.

Each well in 200 reactions format contains 2 nmol of lyophilized primer pair. Please re-dissolve in 200ul of water so the final concentration is 10uM. Each well in the PCR plate contains 10pmol of primer pairs. The primers will be re-suspended once the PCR reagents are added to the plate.

A gene specific qPCR template standard is a tube of linear DNA made from a full-length cDNA plasmid. The copy number of a template standard solution is determined by the PicoGreen method and calculation based on MW.

Yes, as long as a corresponding probe is used.

Yes, as long as it is diluted in the qPCR detection range and each dilution is mixed thoroughly.

Yes, as long as the buffer has no negative effects on qPCR.

Yes. Please contact our Tech Support at techsupport@origene.com

OriGene qPCR primers and templates are warranted for SYBR Green qPCR experiments. If your experimental results are not satisfactory, our scientists will work with you to pinpoint the problem and, if it is determined that our products are at fault, OriGene will refund your money in the form of a credit.

If the cDNA templates in your samples are single-stranded such as cDNA from RT reactions, the actual copy number of your gene of interest is two times the number you got by comparing to the qPCR standards. For example, if the copy number of your gene is 5 copy/μl from the standard curve of a qPCR program, the actual number is 10 copy/μl. The reason is that the first cycle for a single-stranded sample is to make the complementary strand; therefore, there is one cycle delay in the PCR reaction compared to the double-stranded cDNA template standard’s reaction. If the templates in your samples are double-stranded, the copy number of your gene of interest is the same as that calculated according to the qPCR standards.

Here is our guideline:

Full Product Name, OriGene Technologies Inc., Rockville, MD, USA, Catalog #, Lot #.

In addition, we would love to hear from you when the paper is published. Email us a copy of the accepted manuscript and receive a special gift.