First-Strand cDNA FAQs

The cDNAs are synthesized by reverse-transcription of polyA+ RNAs using oligo-dT primer. The polyA+ RNAs are prepared from total RNAs by double purification with oligo-dT affinity method. This procedure greatly enriches the mRNA and increases the chances for successful cDNA amplification of rare transcripts.

For orders of cDNA from individual organs, three 20ul (~5ng/ul) vials of cDNA samples are included in one kit, along with primer pairs of beta-actin for positive control. Each order is enough for 30 reactions.
For orders of a cDNA set of six tissues, one 20ul (~5ng/ul) vial of cDNA from each tissue is included in each kit, along with primer pairs of beta-actin and cyclophilin for positive control. Each order is sufficient for 10 reactions for each tissue.

Here is our guideline:

Full Product Name, OriGene Technologies Inc., Rockville, MD, USA, Catalog #, Lot #.

In addition, we would love to hear from you when the paper is published. Email us a copy of the accepted manuscript and receive a special gift.